Table 1. Comparison of identified tryptic peptides from particle elute and starting solution by nano reverse phase liquid chromatography mass spectrometry (RPLC-MS/MS).
| Dilution | PDGF concentration (ng/ml) | Identified tryptic peptides from Particle Elute a | Peptide Ions b | Peptide Hits c | Identified tryptic peptides from Starting Solution a | Peptide Ions b | Peptide Hitsc |
| 1∶60 | 667 | K.KATVTLEDHLACK.C | 18/24 | 33 | K.KATVTLEDHLACK.C | 17/24 | 13 |
| K.KATVTLEDHLACK.C | 18/24 | K.KATVTLEDHLACK.C | 18/24 | ||||
| K.ATVTLEDHLACK.C | 16/22 | K.ATVTLEDHLACK.C | 16/22 | ||||
| K.KATVTLEDHLACK.C | 19/24 | K.TRTEVFEISR.R | 16/18 | ||||
| K.KATVTLEDHLACK.C | 27/48 | K.TRTEVFEISR.R | 15/18 | ||||
| K.KATVTLEDHLACK.C | 18/24 | K.ATVTLEDHLACK.C | 17/22 | ||||
| K.TRTEVFEISRR.L | 13/20 | K.TRTEVFEISR.R | 16/18 | ||||
| K.ATVTLEDHLACK.C | 16/22 | K.TRTEVFEISR.R | 22/36 | ||||
| K.TRTEVFEISR.R | 17/18 | R.TEVFEISR.R | 13/14 | ||||
| K.ATVTLEDHLACK.C | 17/22 | R.SLGSLTIAEPAMIAECK.T | 21/32 | ||||
| K.ATVTLEDHLACK.C | 15/22 | R.TNANFLVWPPCVEVQR.C | 19/30 | ||||
| K.TRTEVFEISR.R | 15/18 | R.TNANFLVWPPCVEVQR.CR.TNANFLVWPPCVEVQR.C | 22/30 | ||||
| K.ATVTLEDHLACK.C | 17/22 | 22/30 | |||||
| R.NVQCRPTQVQLRPVQVR.K | 26/64 | ||||||
| K.TRTEVFEISR.R | 16/18 | ||||||
| K.TRTEVFEISR.R | 23/36 | ||||||
| K.ATVTLEDHLACK.C | 14/22 | ||||||
| K.TRTEVFEISR.R | 17/18 | ||||||
| K.ATVTLEDHLACKCETVAAAR.P | 34/76 | ||||||
| R.TEVFEISR.R | 13/14 | ||||||
| R.SLGSLTIAEPAMIAECK.T | 15/32 | ||||||
| R.SLGSLTIAEPAMIAECK.T | 21/32 | ||||||
| R.SLGSLTIAEPAMIAECK.T | 41/64 | ||||||
| R.TNANFLVWPPCVEVQR.C | 18/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 22/30 | ||||||
| R.LIDRTNANFLVWPPCVEVQR.C | 32/76 | ||||||
| R.TNANFLVWPPCVEVQR.C | 23/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 23/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 17/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 13/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 15/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 14/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 16/30 | ||||||
| 1∶600 | 66.7 | K.KATVTLEDHLACK.C | 19/24 | 15 | K.KATVTLEDHLACK.C | 18/24 | 6 |
| K.ATVTLEDHLACK.C | 13/22 | R.NVQCRPTQVQLRPVQVR.K | 26/64 | ||||
| K.KATVTLEDHLACK.C | 19/24 | K.TRTEVFEISR.R | 15/18 | ||||
| K.ATVTLEDHLACK.C | 16/22 | R.TEVFEISR.R | 13/14 | ||||
| K.ATVTLEDHLACK.C | 14/22 | R.TNANFLVWPPCVEVQR.CR.TNANFLVWPPCVEVQR.C | 16/30 | ||||
| K.TRTEVFEISR.R | 16/18 | 20/30 | |||||
| K.ATVTLEDHLACK.C | 16/22 | ||||||
| R.NVQCRPTQVQLRPVQVR.K | 28/64 | ||||||
| K.TRTEVFEISR.R | 15/18 | ||||||
| R.TEVFEISR.R | 13/14 | ||||||
| R.SLGSLTIAEPAMIAECK.T | 21/32 | ||||||
| R.TNANFLVWPPCVEVQR.C | 19/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 19/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 21/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 21/30 | ||||||
| 1∶6,000 | 6.67 | K.ATVTLEDHLACK.C | 16/22 | 5 | R.NVQCRPTQVQLRPVQVR.K | 24/64 | 1 |
| R.NVQCRPTQVQLRPVQVR.K | 27/64 | ||||||
| K.TRTEVFEISR.R | 15/18 | ||||||
| R.TNANFLVWPPCVEVQR.C | 14/30 | ||||||
| R.TNANFLVWPPCVEVQR.C | 20/30 | ||||||
| 1∶60,000 | 0.667 | K.ATVTLEDHLACK.C | 13/22 | 1 | PDGF was not detected | 0 |
a
The amino acids between the dots are the identified tryptic peptide sequence, and the amino acid before or after the dot is the upstream or downstream residue of this peptide.
b
Peptide Ions is the ratio of number of matched b-ions and y-ions to the number of theoretical b-ions and y-ions for the identified peptide based on Sequest database search result.
c
Peptide Hits is the total number of identified peptides (including the same peptide with the same charge, the same peptide with different charge, and the different peptide) from one protein based on Sequest database search result.