Table 1.
Up-regulated expression of proteins and their biological functions after acetic acid exposure to Ralstonia eutropha. Results are LC-MS/MS data processed with Mascot search engine and the homology alignments. Uniprot and TIGR classification were used to search cellular roles of identified proteins.
| Protein name | No. of matched peptides | Mascot Score (value P=0.05) | Accession number | Species | Biological function |
|---|---|---|---|---|---|
| Possible proteins involved in PHA production | |||||
| Acetoacetate metabolism regulatory protein | 3 | 32 (27) | Q06065 | Escherichia coli | Transcription |
| Acetoacetyl-CoA reductase | 1 | 78 (33) | RDALAE or P14697 | Ralstonia eutrophus | PHA metabolism |
| Putative acetyl-CoA:acetoacetyl CoA transferase | 3 | 33 (31) | Q8ZPR5_SALTY | Salmonella typhimurium | PHA metabolism |
| Myo-inositol catabolism iolD Probable malonic semialdehyde oxidative decarboxylase | 5 | 35 (21) | P42415 | Bacillus subtilis | Acetyl-CoA biosynthesis (propionyl-CoA metabolism) |
| Methylmalonyl-CoA mutase large subunit | 6 | 42 (25) | P11653 | Propionibacterium freudenreichii shermanii | Propionic acid fermentation |
| Carnitine O-acetyltransferase | 2 | 30 (29) | G90608 | Mycoplasma pulmonis | β-oxidation pathway |
| Probable acyl-CoA dehydrogenase FadE22b | 3 | 43 (26) | Q7TXC4_MYCBO | Mycobacterium bovis | β-oxidation pathway |
| 3-oxoacyl-acyl-carrier protein synthase (FabH) | 2 | 27 (26) | F69842 | Bacillus subtilis | Fatty acid biosynthesis |
| Enoyl-[acyl-carrier-protein] reductase (FabI) | 4 | 27 (23) | P54616 | Bacillus subtilis | Fatty acid biosynthesis |
| Fatty acid/phospholipid synthesis protein | 2 | 32 (23) | Q7NAZ1 | Mycoplasma gallisepticum | Fatty acid biosynthesis |
| Probable fatty acid-CoA ligase FadD30 | 4 | 29 (26) | Q7U226_MYCBO | Mycobacterium bovis | Fatty acid biosynthesis |
| Acetyl/propionyl-CoA carboxylase, beta subunit | 2 | 32 (27) | Q9L077_STRCO | Streptomyces coelicolor | Fatty acid biosynthesis |
| Energy metabolism | |||||
| Acetate kinase | 1 | 22 (22) | Q7NAZ6 | Mycoplasma gallisepticum | Acetyl-CoA biosynthesis |
| Enolase | 5 | 34 (18) | Q8DPS0 | Streptococcus pneumoniae | Energy metabolism |
| Hpr kinase/phosphrylase | 5 | 36 (31) | Q93FD2 | Lactobacillus delbrueckii subsp. bulgaritus | Regulation of carbon metabolism |
| Amino acid biosynthesis | |||||
| Ketol-acid reductoisomerase (Acetohydroxy-acid isomeroreductase) | 5 | 24 (16) | Q8UDV0 | Agrobacterium tumefasciens | Amino acid biosynthesis |
| D-isomer specific 2-hydroxyacid dehydrogenase | 11 | 33 (26) | Q5HW94_CAMJR | Campylobacter jejuni | Amino acid (L-serine) biosyntehsis |
| 5-methyl tetrahydropteroyltriglutamate--homocysteine methyltransferase | 4 | 28 (25) | Q8FQB2 | Streptomyces coelicolor | Methionine biosynthesis |
| Methionine synthase | 6 | 27 (22) | O33259 | Mycobacterium tuberclosis | Methionine biosynthesis |
| Glutamate 5-kinase | 3 | 35 (34) | Q7N7B2 | Photorhabdus luminescens subsp. laumondii | Proline biosynthesis |
| Putative cystathionine gamma-lyase | 2 | 29 (21) | Q59829 | Streptomyces coelicolor | Cysteine biosynthesis |
| Acetylornitine aminotransferase | 2 | 21 (17) | Q8UI71 | Agrobacterium tumefaciens | Arginine biosynthesis |
| Pyrimidine biosynthesis | |||||
| Quinolinate synthetase A | 2 | 24 (17) | Q9F364 | Streptomyces coelicolor | NAD biosynthesis |
| Bifunctional purine biosynthesis protein purH | 7 | 33 (19) | Q9JZM7 | Neisseria meningitidis | Nucleotide biosynthesis |
| Nicotinate phosphoribosyltransferase | 4 | 19 (16) | Q8UIS9 | Agrobacterium tumefaciens | NAD biosynthesis |
| Cofactor biosynthesis | |||||
| CinA-like protein | 3 | 33 (27) | Q67NW5 | Symbiobacterium thermophilum | Biosynthesis of molybdopterin cofactor |
| Coenzyme PQQ synthesis protein E | 3 | 35 (34) | Q01060 | Enterobacter agglomerans | Iron ion binding |
| Lipoyl synthase | 3 | 23 (16) | Q8UFG1 | Agrobacterium tumefaciens | Lipoate biosynthesis |
| Probable phosphoketolase | 4 | 25 (25) | Q5Z066 | Norcadia farcinica | Thiamine biosynthesis |
| Dethiobiotin synthetase | 1 | 19 (17) | Q9FCC1 | Streptomyces coelicolor | Cofactor biosynthesis |
| Stress response proteins | |||||
| Molecular chaperone GroEL | 1 | 71 (44) | Q75T66_BURPI | Burkhoderia picketii | Stabilize or protect disassembled polypeptides |
| Peroxidase/catalase | 3 | 21 (17) | Q9RJH9 | Streptomyces coelicolor | Response to oxidative stress |
| S-adenosylmethionine synthetase | 2 | 31 (25) | Q9X4Q2 | Streptomyces spectabilis | Methyl cycle and polyamine biosynthesis |
| Signal recognition particle protein (fifty-four homolog) | 4 | 25 (22) | P66844 | Mycobacterium tuberclosis | Signal transduction |
| Autoinducer synthesis protein solI | 2 | 35 (34) | P58584 | Ralstonia solanacearum | Signal transduction |
| Thioredoxin reductase | 2 | 22 (22) | P47348 | Mycoplasma gallisepticum | Oxidoreductase |
| Hyaluronate lyase precursor | 4 | 19 (18) | Q54873 | Streptococcus pneumoniae | invasive capacity of the pathogen |
| Toxic anion resistance protein homolog | 3 | 27 (26) | B69757 (MSDB) | Bacillus subtilis | Defense |
| Formate detoxification proteins | |||||
| FdhD protein homolog | 3 | 31 (17) | Q9ZBW0 | Streptomyces coelicolor | Formate dehydrogenase |
| Formate dehydrogenase homolog | 3 | 40 (26) | A27286 | Bacillus subtilis | Formate dehydrogenase |
| Probable Ni/Fe hydrogenase small chain | 4 | 28 (26) | G81284 | Campylobacter jejuni | Electron transport |
| Others | |||||
| Betaine aldehyde dehydrogenase | 5 | 17 (16) | Q8UH56 | Agrobacterium tumefaciens | Bataine biosynthesis Oxidoreductase |
| ComF operon protein | 6 | 29 (23) | P39145 | Bacillus subtilis | ATP binding |
| Phosphate import ATP-binding protein | 4 | 28 (23) | P75186 | Mycoplasma pneumoniae | Transporter |