Table 2.
Up-regulated expression of proteins and their biological functions after propionic acid exposure to Ralstonia eutropha. Results are LC-MS/MS data processed with Mascot search engine and the homology alignments. Uniprot and TIGR classification were used to search cellular roles of identified proteins.
| Protein name | No. of matched peptides | Mascot Score (value P=0.05) | Accession number | Species | Biological function |
|---|---|---|---|---|---|
| Possible proteins involved in PHA production | |||||
| Acetyl-CoA reductase | 1 | 120 (43) | RDALAE | Ralstonia eutropus | PHA metabolism |
| Probable enoyl-CoA hydratase | 4 | 27 (26) | B70695 | Mycobacterium tuberclosis | PHA metabolism |
| Probable acyl-CoA dehydrogenase FadE25 | 3 | 31 (23) | P63427 | Mycobacterium tuberclosis | β-oxidation |
| Probable multi-domain beta keto-acyl synthase | 4 | 37 (27) | T37056 | Streptomyces coelicolor | Fatty acid biosynthesis |
| Acetyl/propionyl-CoA carboxylase | 3 | 30 (26) | P46392 | Mycobacterium | Fatty acid biosynthesis |
| Energy metabolism | |||||
| Enolase 2 | 1 | 19 (17) | Q9F3P9 | Streptococcus coelicolor | Energy metabolism |
| Transaldolase B | 4 | 41 (23) | P66955 | Salmonella typhimurium | Pentose pathway |
| Amino acid biosynthesis | |||||
| Glutamine synthetase | 7 | 26 (23) | P0A590 | Mycobacterium tuberclosis | Amino acid biosynthesis |
| Putative cystathionine gamma-lyase | 1 | 19 (17) | Q59829 | Streptococcus coelicolor | Cysteine biosynthesis |
| Ketol-acid reductoisomerase 2 (Acetohydroxy-acid isomeroreductase) | 4 | 19 (17) | Q9FBR8 | Streptomyces coelicolor | Amino acid biosynthesis |
| 5-methyl tetrahydropteroyltriglutamate--homocysteine methyltransferase | 2 | 34 (29) | O67606 | Aquifex aeolicus | Methionine biosynthesis |
| Histidinol dehydrogenase | 4 | 17 (16) | Q9PM77 | Campylobacter jejuni | Amino acid biosynthesis |
| Carbamate kinase-like protein | 4 | 34 (26) | P77624 | Escherichia coli | Amino acid biosynthesis |
| Histidinol-phosphate aminotransferase | 3 | 29 (17) | Q8U9W3 | Agrobacterium tumefaciens | |
| Purine and pyrimidine biosynthesis | |||||
| Probable xanthine dehydrogenase | 4 | 25 (24) | O32144 | Bacillus subtilis | Purine catabolism |
| Probable inositol monophosphatase | 5 | 29 (27) | T35932 | Streptomyces coelicolor | Aromatic acid biosynthesis (chorismate biosynthesis) |
| Quinolinate synthetase A | 2 | 20 (16) | Q9F364 | Streptomyces coelicolor | NAD biosynthesis |
| NAD-dependent deacetylase (Regulatory protein Sir2) | 3 | 26 (17) | Q9JN05 | Campylobacter jejuni | Transcription |
| Cofactor biosynthesis | |||||
| Biotin synthase | 2 | 24 (23) | P12678 | Salmonella typhimurium | Biotin biosynthesis |
| Stress response proteins | |||||
| Glutathione biosynthesis bifunctional protein | 3 | 41 (31) | Q8DW15 | Streptococcus mutans | Glutathione biosynthesis |
| Sigma-70 | 3 | 32 (25) | F81375 | Campylobacter jejuni | Transcription |
| Signal recognition particle protein (sigma-54) | 2 | 22 (22) | Q01442 | Mycoplasma mycoides | Transcription |
| Alkyl hydroperoxide reductase | 2 | 72 (43) | Q7VTI5_BORPE | Bordetella pertussis | oxidoreductase |
| Gluconate operon transcription repressor | 2 | 24 (23) | P10585 | Bacillus subtilis | Transcription |
| Catalase | 4 | 28 (27) | Q50474 | Mycobacterium tuberclosis | Defense |
| Catalase 2 | 2 | 24 (23) | P42234 | Bacillus subtilis | Defense |
| Formate detoxification proteins | |||||
| Hydrogenase expression/formation protein | 3 | 33 (25) | Q5HVE5_CAMJR | Campylobacter jejuni | Transcription |
| FdhD protein | 3 | 24 (23) | P64118 | Mycobacterium coelicolor | Formate dehydrogenase |
| Transpoters | |||||
| H+-transporting two-sector ATPase | 2 | 29 (27) | Q97PT4_STRPN | Streptococcus pneumoniae | Transporter |
| Potassium-transporting ATPase B chain | 4 | 50 (29) | Q9R6X1 | Anabaena sp. (strain L31) | Transporter |
| H+/K+-exchanging ATPase | 3 | 30 (27) | T36652 | Streptomyces coelicolor | Transporter |
| p-hydroxybenzoic acid-efflux pump subunit | 3 | 26 (26) | Q8FD51 | Escherichia coli | Transporter |
| Others | |||||
| 2,3-dihydroxyphenylpropionate 1,2-dioxygenase | 2 | 30 (26) | P54711 | Escherichia coli | 3-hydroxyphenyl propionate metabolism |
| L-2,4-diaminobutyric acid acetyltransferase | 2 | 19 (17) | Q93RW2 | Streptococcus coelicolor | Polyamine biosynthesis |
| Ethanolamine ammonia-lyase | 1 | 24 (23) | Q8Z4U3 | Salmonella typhimurium | Ethnolamine utilization |
| Cyanate hydratase | 2 | 35 (30) | Q59948 | Synechococcus sp. | Cyanate metabolism |
| Hyaluronate lyase precursor | 5 | 20 (19) | Q54873 | Streptococcus pneumoniae | invasive capacity of the pathogen |
| Desaturase-related protein | 3 | 28 (27) | Q8VK28_MYCTU | Mycobacterium tuberclosis | Not known |