Figure 1. DARPP-32-positive striatal neurons have characteristic nuclear TO-PRO-3 staining.

Confocal image of a striatal neuron triply stained with antibodies for DARPP-32 (green, 1), the nuclear marker TO-PRO-3 (blue, 2) and antibodies for the nucleolar protein fibrillarin (red, 3). Compact chromatin intensely stained with TO-PRO-3 (arrows) is located at the vicinity of nucleoli (arrowheads), but does not overlap with fibrillarin immunoreactivity. Scale bar: 5 µm.