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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: J Biol Chem. 2004 Apr 15;279(27):28209–28219. doi: 10.1074/jbc.M403553200

Fig. 6. NPM overexpression enhances MnSOD mRNA expression.

Fig. 6

HepG2 cells were transfected with 0.5 μg of NPM expression vector (pcDNA3.1/NPM) or empty vector (pcDNA3.1) for 24 h. Cells were then washed with 1x PBS, and total RNA was isolated by the TRIzol method. Thirty μg of RNA was subjected to 1.1% agarose gel electrophoresis and transferred onto nylon membrane. Northern analysis of RNA isolated from control and overexpressing cells were performed using a human MnSOD cDNA probe. The 1- and 4-kb human MnSOD transcripts are indicated by arrows. The same membrane was stripped and re-probed with a human IL-8 cDNA probe (middle arrow) and a β-actin cDNA probe (bottom arrow). The 4-kb MnSOD transcript bands were densitometrically scanned and normalized with β-actin. Statistical analyses were performed using three sets of experiments. Each column represents the mean of three samples ± S.D. **, p < 0.01 as compared with empty vector-transfected control.