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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: J Biol Chem. 2004 Apr 15;279(27):28209–28219. doi: 10.1074/jbc.M403553200

Fig. 7. Antisense NPM overexpression suppresses MnSOD gene induction.

Fig. 7

HepG2 cells were transfected with antisense NPM (pCMV/ASNPM) or empty vector (pCMV) along with MnSOD promoter-and enhancer-driven luciferase reporter vectors. After 12 h of co-transfection, cells were washed, grown for 24 h, and then treated with PMA and cytokines for 12 h, after which cells were again washed. Cell lysates were collected, and the luciferase activity was measured as a determinant of MnSOD gene transcription. Each data point represents the mean of three independent experiments ± S.D. Significant difference from corresponding control: **, p < 0.01. Significant difference from corresponding treatment group: #, p < 0.01.