Table 1. Active Site Interactions, Including Protein−AppN Interactions, Protein−Nick Interactions, and Interactions with the Mg2+-Coordinated Water Molecules.
| length (Å)[standard deviation (Å)] | |
|---|---|
| AppN adenine−F209 phenyl group | 4.14 (0.22) |
| E86 O−AppN adenine N6 | 2.97 (0.20) |
| E159 OE1−AppN ribose O2′ | 2.55 (0.08) |
| N92 ND2−AppN ribose O2′ | 3.30 (0.41) |
| K87 NZ−AppN O1P | 3.39 (0.42) |
| K307 NZ−AppN O2P | 3.87 (0.56) |
| R111 NH1−AppN O1P | 2.80 (0.11) |
| nick 3′-HO−nearest nonbridgingnick 5′-PO4 oxygen | 1.90 (0.64) |
| R111 CZ−nick O3′ | 3.95 (0.34) |
| H89 ND1−WAT O | 2.85 (0.21) |
| E159 CD−WAT O | 3.49 (0.27) |
| E283 CD−WAT O | 3.45 (0.24) |
| E283 CD−WAT O | 3.44 (0.21) |
| nick O2′−WAT O | 3.30 (0.53) |