Table 2. Resorufin Formation (H2O2 formation) and cyt c Reduction (O2•− formation) in the Presence of Hydrophilic Quinones Depend on the Presence of Asolectin, Rotenone, and O2a.
| Resorufin Formation (nmol min−1 mg−1) | |||||||
|---|---|---|---|---|---|---|---|
| Aso | Rot | O2 | CAT | DQ | Q1 | Q0 | IDE |
| + | − | + | − | 33.9 ± 0.1 | 303.8 ± 21.0 | 29.7 ± 3.2 | 119.7 ± 1.3 |
| + | + | + | − | 29.4 ± 6.6 | 234.6 ± 1.2 | 26.2 ± 0.8 | 123.0 ± 0.3 |
| − | − | + | − | 40.9 ± 4.6 | 307.7 ± 4.4 | 38.0 ± 2.5 | 246.6 ± 11.4 |
| − | + | + | − | 41.5 ± 2.0 | 300.1 ± 32.8 | 38.5 ± 5.7 | 257.8 ± 12.9 |
| − | + | + | + | 3.6 ± 0.3 | 23.0 ± 1.2 | 16.0 ± 0.7 | 23.5 ± 2.3 |
| − | + | − | − | 1.0 ± 0.4 | 24.9 ± 1.6 | 9.6 ± 0.8 | 25.4 ± 3.6 |
| Cyt c Reduction (nmol min−1 mg−1) | |||||||
|---|---|---|---|---|---|---|---|
| Aso | Rot | O2 | SOD | DQ | Q1 | Q0 | IDE |
| − | + | + | − | 48.4 ± 3.3 | 532.0 ± 32.6 | 276.8 ± 5.0 | 432.7 ± 3.0 |
| − | + | + | + | 6.4 ± 2.5 | 203.4 ± 6.8 | 125.7 ± 2.5 | 208.6 ± 18.3 |
| − | + | − | − | 26.2 ± 3.0 | 609.0 ± 14.6 | 315.3 ± 4.7 | 461.6 ± 9.5 |
| − | + | − | + | 24.3 ± 0.9 | 552.4 ± 5.2 | 263.1 ± 1.9 | 419.5 ± 23.2 |
a
Conditions: 20 mM Tris-HCl, pH 7.55, 32 °C, 30 μM NADH, 100 μM Q, +O2 = atmospheric O2, −O2 = anaerobic glovebox, Aso = 0.4 mg/mL asolectin, Rot = 2.3 μM rotenone, or 10 μM rotenone for IDE, CAT = 10000 units/mL catalase, SOD = 50 units/mL Cu-Zn-SOD, 50 μM cyt c, 2 units/mL HRP, 10 μM Amplex Red.