Inhibiting Pcdh1 mRNA expression results in an increase in sensory neuron differentiation. Chick embryos electroporated with the Pcdh1-specific or control siRNA, along with a GFP-expressing vector to visualize electroporated neural crest cells (green), then fixed and immunostained for Islet-1 expression (red). Arrows indicate double-labeled cells. D) Quantification of the percentage of double-positive cells in the DRG. The Pcdh1 siRNA results in a greater percentage of GFP/Islet-1 double-positive cells in the DRG (A), as compared to the control siRNA (C), and this phenotype can be rescued by co-injecting the Pcdh1 siRNA along with a vector encoding full-length Pcdh1 (B). * Indicates a statistically significant difference (p<0.01). NT, neural tube.