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EcoRI cross-links to γ-HOPdG positioned within the EcoRI restriction site. 32P-labeled γ-HOPdG-modified or unmodified DNA substrates (160 pM) were incubated with EcoRI (1.6 nM) at room temperature for 2 h in the presence or absence of NaCNBH3. γ-HOPdG was positioned in either the EcoRI restriction site (GAATTC) or a scrambled sequence (GTATAC). The products were resolved by SDS-PAGE. A representative autoradiogram of the gel is shown.
