Figure 4.

Incorporation of ¹⁴C from [¹⁴C]fructose by a mutant carrying ptsG-F and [ΔptsH ptsI crr]KanR. Cells were grown overnight on 20 mM fructose, harvested, and washed. Suspensions (A_{600 nm} = 1) were incubated with 5 mM [¹⁴C]fructose to which was added water (□), 1 mM 2DG (○), 1 mM αMG (●), or 5 mM glucose (▴). Samples were taken at known times, the cells were filtered and washed, and their radioactivity was assayed.