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Negative-stain electron micrographs and corresponding fast Fourier transform (FFT) of two-layer patterned sheets of N-strep dodecamer. (A) Self-assembled huge flat sheets piled into 3D stacks. (B) Representative fast Fourier transformation. (C) The red and blue circles in the image suggested two layers sheets slightly arranged in different angle, suggesting that each sheet formed or grew independently and stacked together.
