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. 2008 Jul 30;51(16):4911–4919. doi: 10.1021/jm700969c

Figure 2.

Figure 2

Compound 24 is a PPARγ agonist. (a) HCA7 cells were transfected with 1.8 μg of PPRE luciferase and 0.2 μg of pSVβgal. Cells were treated with 0−2.5 μM 24 or troglitazone for 12 h. Luciferase activity represents data that have been normalized to β-galactosidase. Columns, means; bars, SD; n = 6. *: p < 0.05, **: p < 0.01. (b) 24 binds to PPARγ. Competitive binding assays were performed by scintillation proximity assay for human PPARγ-ligand binding domain and 50 nM 3H-troglitazone in the presence of increasing concentrations of nonradioactive 24 as a competitor. Means and SD are shown, n = 3.