Table 2. Experimental determinations of minimum crystal size.

This table lists values for n ₀ determined from the scattering power of crystals for which the minimum size required for a complete data set has been reported. The parameters listed in the first two rows are examples that both use the same value of n ₀ for equation (3) and demonstrate that the size requirement of different crystal types can still be governed by a single n ₀ parameter. Note that n ₀ appears to be restricted to a relatively small range when compared with the variety of molecular weights and crystal sizes shown, and that n ₀ has been decreasing over time, perhaps as instrumentation and algorithms have improved. Footnotes indicate derived parameters and an asterisk (*) indicates that equation (4) was used to estimate the Wilson B factor. A question mark (?) indicates that the parameter was not provided in the given reference, but a reasonable average value for protein crystals was substituted.

MW (kDa)	Resolution (Å)	VM (Å3 Da−1)	Wilson B (Å2)	Crystal size (µm)	No. of crystals	n0	Reference
14	1.5	2.0	20	20	1	3.1	Example
100	2.5	2.4	40	15	1	3.1	Example
62†	1.9	2.4?	20*	30	13	130	Gonzalez & Nave (1994 ▶)
14	1.6	2.0	22*	35	1	25	Teng & Moffat (2000 ▶)
28	2.1	2.5	30	20	1	12	Glaeser et al. (2000 ▶)
24	2.0	2.5	22	5 × 30 × 30	5	9.8	Facciotti et al. (2003 ▶)
400	3.5	2.5	65*	20	1	9.3	Sliz et al. (2003 ▶)
28.6	1.98	1.58	11	5	2	5.2	Coulibaly et al. (2007 ▶)
0.8	1.3	1.5	10	1.5 × 1.5 × 5	3	3.7	Nelson et al. (2005 ▶),Sawaya et al. (2007 ▶)
78	2.65	3.06	56	16‡ × 5 × 5	4	3.6	Li et al. (2004 ▶)
73	3.4	3.67	69	5	13	3.2	Standfuss et al. (2007 ▶)
21	1.5	2.4	11.4	1 × 1 × 20	90	3.1	Moukhametzianov et al. (2008 ▶)
6000	3.46	3.4	70	70	17	180	Schuwirth et al. (2005 ▶)

^†Estimated for 100 Å unit cell in P4₃2₁2 with V _M = 2.4.

^‡Taken from 400 µm³ illuminated volume quoted by Moukhametzianov et al. (2008 ▶) and 5 µm beam.