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. 2009 Jan 7;37(4):1127–1140. doi: 10.1093/nar/gkn1020

Figure 6.

Figure 6.

The N-terminal PIN domain in Rrp44 harbors endonuclease activity in vivo. (A) Northern analysis of pre-rRNA processing in the GAL::rrp44 strain transformed with plasmids expressing either WT or mutant Rrp44 protein, or an empty vector pRS315. The mutants analyzed are rrp44-exo, rrp44-endo and rrp44-endo–exo (see Figure 5). RNA was isolated from GAL::rrp44 strains grown at 30°C under permissive conditions (GAL) and 10 h after transcriptional repression (GLU). RNA was separated on an 8% polyacrylamide/8 M urea gel and analyzed as described in Figure 1D. (B) Northern analysis of pre-rRNA processing in rrp44Δ (lanes 1–3) or rrp44Δrrp6Δ (lanes 4–6) strains transformed with a plasmid expressing either WT or mutant Rrp44 protein. Strains were grown at 30°C (rrp44Δ) or 25°C (rrp44Δ rrp6Δ) and RNA was analyzed as in (A). Two different exposures are shown for the probe recognizing precursors of the 5.8S rRNA (#020).