. 2009 Jan 7;37(4):1202–1210. doi: 10.1093/nar/gkn1040

Table 1.

Effects of aminoacylation with phenylalanine and anticodon substitution on k_off (min⁻¹ × 10³) from the ribosomal A site

tRNA	GAA anticodon		GAC anticodon
	Deacyl-	Phe-	Deacyl-	Phe-
tRNA₂^Ala	>500^a	9.1 (2.3)	30 (±6)^a	4.4 (0.4)^b
tRNA₂^Arg	38 (5)	6.7 (1.6)	4.5 (0.8)	7.0 (1.6)
tRNA₂^Glu	120 (39)	5.1 (1.9)	4.4 (1.6)	3.7 (1.0)
tRNA₃^Gly	27 (8)	5.6 (1.7)	3.0 (0.7)	4.9 (1.2)
tRNA₁^Ile	57 (18)	7.3 (1.3)	6.8 (2.3)	4.8 (1.7)
tRNA^Lys	65 (11)	4.5 (1.1)	3.6 (1.0)	3.2 (0.6)
tRNA^Met	73 (17)	7.4 (1.1)	5.2 (1.8)	6.0 (1.5)
tRNA^Phe	110 (39)	8.4 (1.2)	4.1 (1.1)	4.2 (0.3)^b
tRNA₃^Thr	68 (23)	4.8 (1.1)	3.3 (1.0)	5.2 (0.4)^b
tRNA^Trp	13 (5)	4.4 (1.4)	4.3 (1.9)	4.9 (1.7)
tRNA₂^Tyr	37 (8)	7.0 (1.8)	4.5 (1.7)	5.2 (2.1)
tRNA_2A^Val	76 (31)	8.5 (4.0)	6.3 (1.9)	5.1 (0.8)^b

All k_off values were measured with complementary codons. Values (±SD) are for at least three separate experiments. 0.5 min⁻¹ is upper limit of the assay.

^aOlejniczak et al. (3).

^bDale and Uhlenbeck (8).