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Drop test assay to assess the effect of nuclear and mitochondrial expression of MET25p-driven constructs. Targeting of the toxic n125Top1-103 to the nucleus leads to growth inhibition in fermentable (glucose) and nonfermentable (glycerol) growth medium. Targeting of the toxic mt125Top1-103 to mitochondria leads to growth of white colonies in glucose and growth inhibition in glycerol, both of which indicate the formation of respiration deficient ‘petite’ cells.
