Table 1.
Tm values determined from the melting profiles of the fluorescently labelled triplexes shown in Figure 1B
| X | pH | ZY |
|||
|---|---|---|---|---|---|
| AT | TA | GC | CG | ||
| T | 5.0 | 63.8 | 53.8 | 57.8 | 58.1 |
| 5.5 | 54.3 | 39.2 | 43.7 | 44.5 | |
| 6.0 | 39.4 | <28.0 | 27.7 | 28.3 | |
| DMAPdU | 5.0 | 65.4 (64.0) | 54.4 | 59.4 | 58.4 |
| 5.5 | 55.6 | 39.9 | 46.5 | 44.5 | |
| 6.0 | 41.8 | <28.0 | 30.9 | 29.5 | |
| APdU | 5.0 | 66.5 (64.0) | 54.8 | 60.3 | 58.7 |
| 5.5 | 56.9 | 40.3 | 47.0 | 44.9 | |
| 6.0 | 43.7 | <28.0 | 32.3 | 29.9 | |
| GPdU | 5.0 | 67.8 (64.1) | 54.6 | 60.2 | 58.5 |
| 5.5 | 57.7 | 39.6 | 47.2 | 43.9 | |
| 6.0 | 43.8 | <28.0 | 30.7 | 28.4 | |
The Tm values shown in parenthesis are those determined from the annealing profiles of the same triplexes and are included only if there is <1°C difference (hysteresis) with those obtained from melting.