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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: J Surg Res. 2008 Apr 28;150(1):137–143. doi: 10.1016/j.jss.2008.03.047

Figure 5.

Figure 5

Reg I and MKP-1 co-precipitated in RIN cells overexpressing reg I protein. Whole cell lysates were generated from confluent RIN or RIN transfected pcDNA3-regI. Cell lysates were immunoprecipitated with mouse anti-reg monoclonal antibody overnight at 4°C and immunoprecipitates were resolved by SDS-12% PAGE; precipitated proteins were detected by immunoblotting with a bovine anti-MKP-1 and by a mouse anti-reg antibody.