FIG. 4.

Examples of validation data. (A) Plate activity heatmaps of an eight-concentration LOPAC screen repeated three times. Concentrations are shown from lowest to highest. Each plate contains an intra-plate control titration. (B) Activity of validation run shows samples identified as inhibitors (blue), activators (red), or inactives (gray); control titration (green) presents as near-overlapping curves indicating excellent assay stability and reproducibility (control 50% inhibitory concentration = 2 μM, control MSR = 1.9). (C) An example of three different compounds showing excellent triplicate CRC reproducibility: samples A(1) (▪), A(2) (▴), A(3) (▾), B(1) (♦), B(2) (•), B(3) (□), C(1) (▵), C(2) (▿), and C(3) (◊).