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“Busy screen.” The multiple dispense, incubation, and read steps are indicated on the Spotfire (TIBCO Software, Somerville, MA) plots: Dispense (
), Incubate (
), Read (
), Read2 (
), Read3 (
), and Transfer (
). The order and timing of all protocol steps remained stable throughout the screen, as evidenced from the near-identical snapshots obtained from six (A) early and (B) late screening plates.
