FIG. 8.

Effect of cytometer scan resolution on CRC determination. A 1,536-well plate assay for glucocorticoid receptor nuclear translocation was performed by fixing U2OS cells and staining nuclei with propidium iodide. The amount of GFP signal within the nucleus was then measured on the Acumen laser cytometer. Representative images of untreated U2OS cells stained for nuclei (left panels) (red is the propidium iodide channel) or cells (middle panels) (green is the GFP channel; red is the propidium iodide channel) are shown with CRC data (right panels) obtained when scanned at either (A) 1 × 0.5 μm or (B) 1 × 8 μm resolution.