Skip to main content
. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Biochemistry. 2008 Aug 9;47(35):9098–9106. doi: 10.1021/bi800938g

Figure 2.

Figure 2

Binding of oligomeric forms of RS1 to galactose-agarose. Culture medium from HEK 293 cells expressing wild-type, C59S/C223S double mutant or C40S/C59S/C223S triple mutant were incubated with galactose agarose at 4°C for 1 h. After the matrix was washed to remove unbound protein, the bound RS1 was eluted with 4% SDS for analysis by SDS gel electrophoresis and Western blotting. A. Western blots of wild-type (octamer), C59S/C223S (dimer) and C40S/C59S/C223S (monomer) RS1 analyzed on non-reducing SDS gels. B. Binding of wild-type and mutant RS1 to galactose-agarose as analyzed on reducing SDS gels.