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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Biochemistry. 2008 Aug 9;47(35):9098–9106. doi: 10.1021/bi800938g

Figure 3.

Figure 3

The effect of various carbohydrates and temperature on the dissociation of RS1 from galactose-agarose. A. RS1 bound to galactose-agarose was eluted with 1 M saccharide at 25°C for 1 h followed by elution with 4% SDS for analysis on western blots labelled for RS1. The lane at the right side of the panel represents the direct elution with 4% SDS. B. RS1 bound to galactose-agarose was eluted sequentially with IPTG and SDS at the indicated temperature. Top panel shows the effect of IPTG and SDS on the dissociation of RS1 from galactose-agarose. Bottom panel shows the % dissociation of RS1 relative to that obtained by SDS (left lane). Data is for 3 independent experiments +/− SD.