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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Biochemistry. 2008 Aug 9;47(35):9098–9106. doi: 10.1021/bi800938g

Figure 5.

Figure 5

Binding of R141 mutants to galactose-agarose. A. Model showing the location of the R141 residue on spike 3 of the RS1 discoidin domain (model adapted from (9)). B. The secreted fraction of HEK 293 cells expressing wild-type RS1 or R141A, R141S, and R141H mutants were applied to either an anti-RS1 3R10 antibody-Sepharose beads (top panel) or a galactose-agarose beads (bottom panel). After washing to remove unbound protein, bound RS1 was eluted with 4% SDS for analysis by western blotting. The R141A and R141H mutants bound to galactose-agarose at similar levels to wild-type RS1, whereas the R141S mutant showed reduced binding.