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Inhibition of RS1 binding to galactose-agarose by IPTG. Binding of RS1 to galactose-agarose was measured in the presence of increasing concentrations of IPTG. Bound RS1 was quantified by western blotting. The data is fitted with a curve for a single set of binding sites with a Kd of 0.35 M ± 0.06.
