Table 1.

Purification of RS1 from Insect Cell Culture Fluid

Purification step	Total protein	Total RS1	Total RS1/Total protein	Purification
Insect cell supernatant	296 mg	646 μg	0.0022	---
DEAE sephacel elution	7.8 mg	336 μg	0.043	19.5
D-galactose column	117.8 μg	86 μg	0.73	332

Total protein concentration was determined by the method of Bradford. RS1 concentration was determined by western blot against a calibration standard curve of different amounts of highly purified RS1 protein.