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. Author manuscript; available in PMC: 2009 Mar 5.
Published in final edited form as: Microbes Infect. 2007 Oct 2;10(1):21–28. doi: 10.1016/j.micinf.2007.09.017

Figure 4. Immunostaining of mouse embryo cardiac myocyte gap junction protein Cx43.

Figure 4

A and C show the merged image of DIC and anti-Cx43 antibody. Non-infected (A and B) cultures presented abundant gap junction plaques (arrows) between coupled cardiac myocytes. After 72 hours of infection (C and D), heavily infected cells (*) lost Cx43 staining and non-infected cells in the same field displayed strong junction plaques, similar to controls (arrows). Note in C, amastigote forms of the parasite revealed by DIC microscopy. (Scale bars = 50.0μm).