Skip to main content
. Author manuscript; available in PMC: 2009 Dec 1.
Published in final edited form as: Curr Opin Chem Biol. 2008 Oct 18;12(6):667–673. doi: 10.1016/j.cbpa.2008.09.024

Figure 3. Structural changes in the 16S rRNA during late steps of assembly.

Figure 3

For structure mapping, reconstitution intermediate (RI) was formed from 16S rRNA plus recombinant S4-S9, S11-S13, S15-S20 at 4 °C, then shifted to 42 °C (RI*), and chased into 30S complexes with the addition of tertiary assembly proteins (S3, S3, S10, S14, S21) [36]. b. Structural differences between RI and RI* determined by dimethylsulfate base modification (red) and hydroxyl radical cleavage (light blue) [36]. N6,N6-dimethyl A1518 and A1519 products of KsgA are shown in green; blue triangles indicate ΔrimM suppressors [38].