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. 2009 Feb 26;10:27. doi: 10.1186/1471-2474-10-27

Table 1.

Reference gene primer sequences used for qPCR

Gene SCS Efficiency (%) Sequence
GapDH
AF157626 		-3.32 100.2 5'GCATCGTGGAGGGACTCA3'
3'GCCACATCTTCCCAGAGG5'
18S
AJ311673 		-3.24 103.3 5'GGCGTCCCCCAACTTCTTA3'
3'GGGCATCACAGACCTGTTATTG5'
ACTB
AF035774 		-3.33 99.9 (Bogaerts et al., 2006)
5'CCAGCACGATGAAGATCAAG3'
3'GTGGACAATGAGGCCAGAAT5'
SDHA
DQ402987 		-3.29 101.5 5'ACAGAGGAATGGTCTGGAATACTGA3'
3' GTGAGCACCACGTGACTCCTT5'

SCS = Standard Curve Slope