Skip to main content
. Author manuscript; available in PMC: 2009 Jul 3.
Published in final edited form as: Oncogene. 2008 Mar 10;27(29):4065–4074. doi: 10.1038/onc.2008.48

Figure 3. The PIKK hSMG-1 mediates early p53 phosphorylation in hyperoxia.

Figure 3

(a) A549 cells were transfected with siRNA against luciferase, ATM or hSMG-1 and immediately harvested (0 hours) or after 1, 3, 6, and 12 hours of hyperoxia. Cell lysates were immunoblotted for p53, p53 (Ser15) and actin. (b) A549 cells were transfected with siRNA against luciferase, ATM, or hSMG-1 and after 24, 48, and 72 hours of hyperoxia. Cell lysates were immunoblotted for p53, p53 (Ser15) and actin. After normalizing band intensities to levels of actin, the fold induction of p53 (Ser 15) during hyperoxia was determined and listed below each lane. Blots are representative of 3–4 experiments with similar results.