Table 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or description a | Reference |
|---|---|---|
| Plasmids | ||
| pKD46 | repA101ts, oriR101, araC-ParaB-gam-bet-exo, bla | [28] |
| pCP20 | FLP+, λ cl857+ λ pR Repts, AmpR, CmR | [28] |
| pKD3 | pANTSy derivative, FRT-flanked cat from pSC140, CmR | [28] |
| prpoE | rpoE with endogenous promoters in pWSK29, AmpR | This work |
| psrfN-2HA | srfN with endogenous promoter and C-term. tandem HA fusion in pWSK129, KanR | Our collection |
| psseL-2HA | sseL with endogenous promoter and C-term. tandem HA fusion in pWSK129, KanR | [8] |
| psseL-2HA | sifA with endogenous promoter and internal tandem HA fusion in pACYC184 | [37] |
| pIVET5n | tnpR-lacZ, sacB, R6K ori, bla, AmpR | [29] |
| pPsseA-lacZ | sseA promoter fused to tnpR-lacZ in pIVET5n, AmpR | [29] |
| pPsseL-lacZ | sseA promoter fused to tnpR-lacZ in pIVET5n, AmpR | This work |
| pPsrfN-lacZ | srfN promoter fused to tnpR-lacZ in pIVET5n, AmpR | This work |
| pPsifA-lacZ | sifA promoter fused to tnpR-lacZ in pIVET5n, AmpR | This work |
| pPssaB-lacZ | ssaB promoter fused to tnpR-lacZ in pIVET5n, AmpR | This work |
| pPssaG-lacZ | ssaG promoter fused to tnpR-lacZ in pIVET5n, AmpR | This work |
| Strains | ||
| SL1344 | wild type S. enterica sv. Typhimurium, SmR | [38] |
| 14028s | wild type S. enterica sv. Typhimurium | [27] |
| 14028s rpoE::cat | rpoE deletion, CmR | [27] |
| 140282 rpoS::Tn10dCm | rpoS deletion, CmR | [27] |
| 14028s rpoN::Ap | rpoN deletion, AmpR | [27] |
| 14028s rpoH::PCN | rpoH deletion, temperature sensitive, AmpR | [27] |
| SL1344 ΔrpoE | Unmarked, in-frame deletion of rpoE, SmR | This work |
| SL1344 ssrB::3FLAG | ssrB-FLAG replacement allele on chromosome | This work |
| SL1344 PsseA::pPsseA-lacZ | Merodiploid containing integrated PsseA-lacZ reporter | [25] |
| SL1344 PsseL::pPsseL-lacZ | Merodiploid containing integrated PsseL-lacZ reporter | This work |
| SL1344 PsrfN::pPsrfN-lacZ | Merodiploid containing integrated PsrfN-lacZ reporter | This work |
| SL1344 PsifA::pPsifA-lacZ | Merodiploid containing integrated PsifA-lacZ reporter | This work |
| SL1344 PssaB::pPssaB-lacZ | Merodiploid containing integrated PssaB-lacZ reporter | This work |
| SL1344 PssaG::pPssaG-lacZ | Merodiploid containing integrated PssaG-lacZ reporter | This work |
| ΔrpoE PsseA::pPsseA-lacZ | Merodiploid containing integrated PsseA-lacZ reporter | This work |
| ΔrpoE PsseL::pPsseL-lacZ | Merodiploid containing integrated PsseL-lacZ reporter | This work |
| ΔrpoE PsrfN::pPsrfN-lacZ | Merodiploid containing integrated PsrfN-lacZ reporter | This work |
| ΔrpoE PsifA::pPsifA-lacZ | Merodiploid containing integrated PsifA-lacZ reporter | This work |
| ΔrpoE PssaB::pPssaB-lacZ | Merodiploid containing integrated PssaB-lacZ reporter | This work |
| ΔrpoE PssaG::pPssaG-lacZ | Merodiploid containing integrated PssaG-lacZ reporter | This work |
aSm, streptomycin; Amp, ampicillin; Cm, chloramphenicol; Kan, kanamycin