Figure 11.

Microarray results and corresponding RT-PCR results for ZBTB22 and NFAT5 expression in Ad-transformed cells. The microarray results for ZBTB22 indicating up-regulation of the transcript in Ad12 E1-TC were validated by real-time PCR. The microarray result for NFAT5 indicating significant up-regulation in Ad5 E1-TC (1.58-fold; table 1) was validated by real-time PCR. The down-regulation in Ad12 E1-TC indicated by the microarray study was not significant (0.17-fold; table 1) due to high variability (p-value; 0.635) and the real-time PCR results for expression of NFAT5 also did not indicate any significant dysregulation of this transcript in Ad12 E1-TC, or Ad12-TC.