Table 1.
Real-time PCR primer combinations used to amplify genes of interest.
| NCBI accession (cDNA clone) | NCBI accession for mRNA record | Primer | Sequence of primer | Position | Size (bp) |
| AI044043 | NM_001009172.2 | ZBTB22 (F) | 5'-GAGAGCCCGAGGCTACCCT-3' | 1204 – 1222 | 74 |
| ZBTB22 (R) | 5'-CCTGTTCCTCCCCCTTTTCT-3' | 1278 – 1259 | |||
| AI059162 | NM_022605.1 | HPSE (F) | 5'-GGCTCTCCACGGCTTCG-3' | 270 – 286 | 65 |
| HPSE (R) | 5'-GGTGCCGCCAAATCTCAA-3' | 335 – 318 | |||
| AI145841 | NM_199267.2 | RelA (F) | 5'-GGCATGCGTTTCCGTTACA-3' | 152 – 170 | 68 |
| RelA (R) | 5'-ATCTGTGCTTCTCTCCCCAGG-3' | 220 – 200 | |||
| AI058327 | XM_576071.1 | MLK1 (F) | 5'-ACCCCAGCTGCTACCCG-3' | 359 – 375 | 71 |
| MLK1 (R) | 5'-CGATGATCTCCTCCAGGGTTAG-3' | 430 – 409 | |||
| AA924727 | Z78279.1 | COL1A1 (F) | 5'-AAAACGGGAGGGCGAGTG-3' | 228 – 245 | 80 |
| COL1A1 (R) | 5'-GGTCCCTCGACTCCTATGACTTC-3' | 308 – 286 | |||
| AI145995 | AY648296.1 | PPP1R1A (F) | 5'-AGACTCAGGAGCAGCGTGGT-3' | 559 – 578 | 65 |
| PPP1R1A (R) | 5'-CCAGTGGTAGCATGTGGGC-3' | 624 – 606 | |||
| AI044118 | NM_001107425.1 | NFAT5 (F) | 5'-CCTTTAAGGATGCGCTCCAA-3' | 13098 – 13117 | 77 |
| NFAT5 (R) | 5'-CAGGGAGGAAAAGAAGAGAGTGAA-3' | 13151 – 13174 |
The NCBI accession numbers for the cDNA clones on the filters used in the initial microarray study are listed with the accession numbers for the corresponding mRNA records identified by bioinformatics analysis. Abbreviations for primers correspond to the gene symbols of the target genes; zinc finger and BTB domain containing 22 (ZBTB22), V-rel reticuloendotheliosis viral oncogene homolog A (avian) (RelA), mixed-lineage kinase 1 (MLK-1; synonym MAP3K9), collagen type 1, alpha 1 (COL1A1), protein phosphatase regulatory (inhibitor) subunit 1A (PPP1R1A) and Nuclear factor of activated T-cells 5 (NFAT5). Positions of the forward and reverse primers within the mRNA sequence are given in base pairs (bp).