Table 1.
Laminar structures and cellular density in control and mutant olfactory bulbs
| Layer width, μm
|
Cell density, cells per 100 × 100 μm
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| GL* | EPL | IPL | GCL | Periglom. | Periglom. BrdUrd+ | Mitral | Granule | Granule BrdUrd+ | |
| WT | 155.2 ± 4.8 | 162.8 ± 6.4 | 45.3 ± 2.1 | 235.9 ± 12.5 | 104.7 ± 3.2 | 5.7 ± 0.4 | 10.7 ± 0.4 | 192.2 ± 11.3 | 4.2 ± 0.1 |
| KO | 147.0 ± 2.8 | 161.9 ± 2.4 | 43.4 ± 1.0 | 153.6 ± 4.9 | 110.9 ± 6.8 | 6.6 ± 0.4 | 11.5 ± 0.6 | 205.3 ± 7.8 | 4.8 ± 0.2 |
| P | 0.14 | 0.95 | 0.35 | <10−5 | 0.42 | 0.10 | 0.26 | 0.34 | 0.12 |
Values represent mean ± SEM and comparisons were analyzed by unpaired Student's t test from sagittal sections [n = 33 and 63 slices from 4 control (WT) and 5 mutant (KO) mice, respectively]. For counts of newly generated cells, three animals of each genotype were analyzed (45 sections per animal). EPL, external plexiform layer; GCL, granule cell layer; GL, glomerular layer; IPL, internal plexiform layer; Mitral, mitral cells; Granule, interneurons from the granule cell layer; Periglom., interneurons from the glomerular layer. These data were obtained from 12-month-old mice.
The mean diameter of glomeruli was not significantly different between both groups (77.1 ± 1.2 μm and 83.6 ± 2.7 μm for normal and mutant olfactory bulbs, respectively; P = 0.11).