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. 2007 Nov 1;66(5):1207–1218. doi: 10.1111/j.1365-2958.2007.05995.x

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© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd

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Fig. 2 — Indentification of S. Typhi flagellar antigens. A. Western blotting of various S. Typhi mid-log phase whole-cell lysates with anti-z66 flagellar antibody. Lane 1, S. Typhi Ty2; Lane 2, S. Typhi Ty2(ΔfliC); Lane 3, S. Typhi 403ty-fliC(j); Lane 4, S. Typhi 404ty-fliC(d); Lane 5, S. Typhi 404ty ΔfljB^z66; Lane 6, S. Typhi 404ty ΔfljA^z66; Lane 7, S. Typhi 404ty ΔfljB^z66, ΔfljA^z66; Lane 8, S. Typhi 403tya-fliC(j) (post-phase switch); Lane 9, S. Typhi 404tya-fliC(d) (post-phase switch). Protein sizes were estimated on SDS-PAGE gels prior to transfer with seeblue2 protein ladder (Invitrogen). B. Western blotting of various S. Typhi whole-cell lysates with anti-d flagellar antibody. Lanes as A. C. Western blotting of various S. Typhi whole-cell lysates with non-specific Salmonella flagellar antibody. Lanes as A. D. RT-PCR detecting mRNA transcription of aroC (lanes a, primers aroC_RT_F/R, 100 bp), fliC (lanes b, primers fliC_RT_F/R 150, bp), fljB^z66 (lanes c, primers fljB_RT_F/R, 200 bp) and fljA^z66 (lanes d, primers fljA_RT_F/R, 250 bp). Panel 1, genomic DNA from S. Typhi 404ty-fliC(d); panel 2, cDNA from S. Typhi Ty2; panel 3, cDNA from S. Typhi 404ty-fliC(d); panel 4, cDNA from S. Typhi 404ty-fliC(d)ΔfljB^z66; panel 5, cDNA from S. Typhi 404ty-fliC(d) ΔfljA^z66 and panel 6, cDNA from S. Typhi 404ty-fliC(d) ΔfljB^z66, ΔfljA^z66. Sizes are compared with the migration of Hyperladder IV (Bioline).