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Residues 1–39 of the N-terminal extension are required for MITS1 to reach mitochondria. (A) A schematic representation of MITS1 lacking the first 39 amino acids. (B) In the absence of the N-terminal pre-sequence, MITS1 was found in the cytosol (empty arrowhead), which in plant cells assumes a diffuse yet reticulated appearance. No co-localization was noticed with the mitochondrial marker, β-ATPase:GFP (arrow). Insets: magnified section of main panels. Scale bars=5 μm.
