Fig. 4.

Detection and identification of immuno-pull-down proteins from OsCAS–YFP and YFP transgenic lines. Immuno-pull-down proteins from these two transgenic lines washed with low stringency buffer were applied for silver staining (A) and immuno-detection of YFP-tagged protein, respectively (B). Arrows point out the differences in patterns of immuno-pull-down proteins between those lines in (A). To further reduce the background of immuno-pull-down proteins, immuno-pull-down proteins from OsCAS–YFP lines washed with high stringency buffer supplied in the kit were visualized by Coomassie blue staining. Four immuno-pull-down proteins were detected and identified (C, labelled with numbers). In a negative control, immobilized anti-GFP antibody was applied for Coomassie blue staining (D).