Fig. 7.

Immunodetection of YFP-tagged protein from transgenic lines and yeast transformants incubated with chemical cross-linker BS³. YFP tag or HIS₆ tag fused with OsCAS from either 40 μg of crude extracts from the transgenic line (A) or 30 μg of crude protein from the yeast transformant (B), respectively, incubated with various concentrations of BS³, were immune-detected with anti-GFP (A) antibody or anti-HIS antibody (B). The arrows indicate monomeric and dimeric forms of OsCAS–YFP (upper panel). Equal loading of crude extracts were visualized by Coomassie blue staining (lower panel).