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. 2009 Mar 11;4(3):e4782. doi: 10.1371/journal.pone.0004782

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Scambi et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Malondialdehyde content of red blood cells at baseline and after supplementation (B) Red cell membrane proteins were separated by mono-dimensional electrophoresis and labelled with fluorescein-5-maleimide, which labels the proteins with active thiol-groups. Fluorescence was detected by placing the gel on a UV light source, photographed and analyzed. CF patients are indicated by numbers from 1 to 11; b: baseline; a: after supplementation with 5-MTHF and vitamin B12. The bands showing different fluorescence are indicated on the colloidal Commassie stained gel with numbers from 1 to 8.