Figure 1.

(A) Deduced amino acid sequences of Ap oa₁ clones from A. californica (A. cal., Upper) and A. kurodai (A. kur., Lower). The coding region is determined as the longest ORF that begins with a methionine codon ATG. The different amino acids are shaded in gray. The putative transmembrane domains are overlined and numbered I–VII. Two potential N-linked glycosylation sites are indicated by open circles (○). Serine and threonine that are within the protein kinase C consensus sequences are indicated by closed circles (●). A potential N-myristoylation site is underlined. (B) Ap oa₁ is expressed in the Aplysia CNS but not in the sensory cluster (SC). Neither Ap oa₁ nor 18S RNA was amplified in CNS when reverse transcriptase was omitted in RT-PCR reaction mixture (CNS-RT).