Figure 5.

Iontophoresis of Rp-cAMPS into the sensory neurons expressing GFP/Ap oa₁. (A) Rp-cAMPS blocked the increase in membrane excitability produced by either Ap oa₁ or endogenous 5-HT receptor. (B) Rp-cAMPS blocked spike broadening produced by Ap oa₁. However, later application of 5-HT produced spike broadening in the presence of Rp-cAMPS. *, P < 0.04 (n = 5), P < 0.002 (n = 9), one-tailed Wilcoxon signed rank test. Expression level of Ap oa₁ was controlled by using different concentrations (10, 50, and 500 ng/μl) of pNEXδ-Ap oa₁ in the microinjection solution. Thick lines under histograms indicate the presence of drugs in the bath, as described in the legend of Fig. 4.