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. 2009 Mar 11;4(3):e4807. doi: 10.1371/journal.pone.0004807

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Monocytes derived from adherence to plastic were plated onto poly-l-lysine coated glass coverslips. The cells were incubated for 30 minutes with mitotracker (panels H, I), and/or fixed, permeabilized, and stained with monoclonal antibodies to AKAP79 (panels A, C, D, F), AKAP149 (panels G, I), AKAP95 (panels J, L), HLA-DR (panels B, C, K, L), Ezrin (panels M, P), RIIβ (panels, E, F, Q, R). Hoescht (panel K, L) was added with secondary antibody conjugates. Panels P-R are 2 fold zoom of the boxed area of panel M. Scale bar = 25 µm.