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. 2009 Mar 11;4(3):e4807. doi: 10.1371/journal.pone.0004807

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Allogeneic day 5 dendritic cells (iDC) were incubated without (Unt, black bars) or with A) the indicated concentrations of type II anchoring inhibitor (IS, red bars) or the control (SCR, blue bars), or B) the indicated concentrations of type I anchoring inhibitor (RIAD, red bars) or the control (RSCR, blue bars) for 1 hr at 37°C, 5% CO₂. The cells were collected, rinsed and replated. Then 0.1 µg/ml of protein antigen (CFP10) was added for 1 hr at 37°C, 5% CO₂, and antigen specific CD4⁺ clonal T cells were added. IFN-γ production was assayed after 16 hours by ELISPOT. Data are an average of three donors. Error bars are S.E.M. Student ttest reveals significance, P<0.05(*).