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. 2009 Mar 12;4(3):e4822. doi: 10.1371/journal.pone.0004822

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Noels et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A, Inclusion of one or two Ig-like domains of MALT1 in MALT1-API2 allows the binding of endogenous BCL10 in HEK-293T. Immunoblot with α-TRAF6, α-BCL10 and α-Flag on protein complexes co-precipitated with Flag-bio-tagged M2A8, M4A8, M7A8, M8A8 and MALT1, transiently expressed in HEK-293T cells. B, M4A8 binds endogenous BCL10 in SSK41 B-cells. Immunoblot with α-BCL10 and α-Flag on protein complexes pulled down with Flag-bio-tagged M4A8 stably expressed in SSK41-BirA B-cells. Precipitated protein complexes from two different SSK41-BirA-M4A8 clones (69 and 93) were compared to the protein complex from HEK-293T cells with transient overexpression of M4A8. Molecular weight standards are in kDa.