Table 2. Differential protein expression of L. hongkongensis at 20°C and 37°C.
Spot no. | Gene no. | Theoretical MW (kDa) | Theoretical pI | Proteins | Functional categories (COG) | Sequence coverage (%) | Peptide matched | MOWSE score | 20°C/37°C ratioa | p value |
Higher expression at 20°C | ||||||||||
1 | LHK_02829 | 30.0 | 5.03 | Acetylglutamate kinase, NAGK-20 | Amino acid transport and metabolism | 24 | 6 | 9.49e+003 | +2.50 | <0.005 |
2 | LHK_01819 | 39.0 | 6.30 | Probable porin protein | Cell wall/membrane/envelope biogenesis | 36 | 9 | 3.86e+005 | +2.41 | <0.005 |
3 | LHK_00236 | 49.5 | 7.06 | Peptidase M16 domain protein precursor | — | 33 | 14 | 2.47e+005 | +2.04 | <0.05 |
Only at 20°C | ||||||||||
4 | LHK_00011 | 16.9 | 4.99 | Protein-L-isoaspartate (D-aspartate) O-methyltransferase | Post-translational modification, protein turnover, chaperones | 41 | 9 | 6.28e+03 | — | <0.01 |
5 | LHK_01152 | 49.0 | 5.44 | Probable phage sheath protein | — | 28 | 10 | 1.11e+005 | — | <0.05 |
6 | LHK_01472 | 23.4 | 4.70 | Ribonuclease activity regulator protein RraA | General function prediction only | 36 | 7 | 4.13e+03 | — | <0.01 |
7 | LHK_01018 | 30.3 | 6.66 | Methylenetetrahydro-folate dehydrogenase/cyclohydrolase | Coenzyme transport and metabolism | 45 | 14 | 6.67e+007 | — | <0.05 |
Higher expression at 37°C | ||||||||||
8 | LHK_02337 | 31.5 | 5.12 | Acetylglutamate kinase, NAGK-37 | Amino acid transport and metabolism | 38 | 13 | 3.48e+004 | −2.09 | <0.005 |
9 | LHK_02119 | 23.7 | 9.22 | Hypothetical protein | — | 41 | 8 | 1.24e+004 | −3.06 | <0.005 |
Only at 37°C | ||||||||||
10 | LHK_02507 | 44.6 | 9.11 | Probable N-acetylmuramoyl-L-alanine amidase | Cell wall/membrane/envelope biogenesis | 58 | 20 | 6.01e+009 | — | <0.001 |
11 | LHK_02507 | 44.6 | 9.11 | Probable N-acetylmuramoyl-L-alanine amidase | Cell wall/membrane/envelope biogenesis | 68 | 23 | 7.6e+010 | — | <0.05 |
12 | LHK_03194 | 48.9 | 7.94 | Survival protein SurA precursor | Posttranslational modification, protein turnover, chaperones | 33 | 10 | 3.79e+006 | — | <0.005 |
A negative number indicates the inverse ratio of that indicated in the column heading.