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. 2009 Mar 13;5(3):e1000417. doi: 10.1371/journal.pgen.1000417

Figure 1. Loading of CPC proteins in prophase I spermatocytes.

Figure 1

(A–D) Early and mid pachytene spermatocytes double immunolabeled for Borealin (green) and INCENP (red). INCENP appears at synaptonemal complex stretches (arrows). Both proteins colocalize at chromocenters (arrowheads) only in mid pachytene. (E–H) Late pachytene and early diplotene spermatocytes double immunolabeled for INCENP (green) and Aurora-B (red). Both proteins colocalize at chromocenters (arrowheads) in early diplotene. The spermatocytes shown are projections of several focal planes, and are counterstained with DAPI (blue). The sex body is indicated (XY). Scale bar 10 µm.