Figure 6. Loading sequence of different inner centromere and outer kinetochore proteins throughout mouse prophase I.

A single telocentric bivalent with homologs in light and dark grey is depicted. The kinetochores, as revealed with the ACA serum, are shown in red, and the lateral elements of the synaptonemal complex are shown in green. The bivalent ends are shown associated to the nuclear envelope (single black line) from early pachytene up to late diplotene. Borealin (pink) is the first CPC protein loaded at the pericentromeric heterochromatin, followed by INCENP (light blue) and Aurora-B (orange). Note that INCENP relocalizes from the central element of the synaptonemal complex to the centromere during mid pachytene. After the CPC complex is loaded, SGOL2 (fuchsia) and MCAK (dark blue) are subsequently recruited to the inner centromere. Finally, the outer kinetochore proteins BubR1 (maroon) and CENP-E (black) assemble onto kinetochores. For details see text.