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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: J Immunol. 2009 Feb 1;182(3):1325–1333. doi: 10.4049/jimmunol.182.3.1325

FIGURE 5.

FIGURE 5

Transfection of miR-513 precursor reduces IFN-γ-induced B7-H1 protein expression in H69 cells. A, miR-513 precursor reduces IFN-γ-induced B7-H1 protein expression. H69 cells were transfected with miR-513 precursor or a control nonspecific precursor for 48 h and then exposed to IFN-γ (10 ng/ml) for 24 h followed by Western blotting for B7-H1. A representative Western blot from three independent experiments and densitometric levels of B7-H1 signals are shown. #, p < 0.05, vs IFN-γ-treated alone. B, miR-513 precursor transfection does not affect IFN-γ-stimulated B7-H1 mRNA expression. H69 cells were transfected with miR-513 precursor or a control nonspecific precursor for 48 h and then exposed to IFN-γ (10 ng/ml) for 24 h followed by real-time PCR analysis for B7-H1 mRNA. *, p < 0.05, vs non-IFN-γ-stimulated control. C, miR-513 precursor transfection does not affect B7-H1 mRNA expression in nonstimulated cells. H69 cells were transfected with miR-513 precursor or a control nonspecific precursor for 48 h in the absence of IFN-γ followed by B7-H1 mRNA real-time PCR analysis.