Figure 2. Testing the stimulation frequency in isolated dorsal roots.
A, recordings of C-fibre CAPCs in isolated dorsal roots (L4, conduction distance, 4.5 mm). Each family of traces has 15 consecutive (individual) recordings of CAPCs activated at 1 Hz and 0.1 Hz. Fast A-components are truncated. B, Aδ-fibre CAPCs in isolated dorsal roots (L5, conduction distance, 10 mm). Each of 15 traces shown at 10 Hz and 1 Hz stimulation is an average of 20 individual traces. In experiments with Aδ-fibre CAPCs, a protocol with 15 stimulations at a given frequency was repeated 20 times (with 4 s intervals between protocols) and the corresponding episodes were averaged. C, examples of mono- and polysynaptic EPSCs recorded in an SG neurone. Each group is a superposition of 10 consecutive recordings. Holding potential was −70 mV. D, examples of recordings from an SG neurone held at −70 and −80 mV. Both EPSCs and IPSCs were seen at −70 mV, whereas only EPSCs were seen at −80 mV.
